蟾酥提取物中2种蟾蜍甾烯类成分兔体内药代动力学研究

刘冬; 杜守颖; 何秀峰; 王爱国; 李娜

doi:CNKI:11-3495/R.20110906.1107.004

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蟾酥提取物中2种蟾蜍甾烯类成分兔体内药代动力学研究

更新时间：2024-01-04

- 蟾酥提取物中2种蟾蜍甾烯类成分兔体内药代动力学研究
- Studies on Simultaneous Determination and Pharmacokinetics of Two Bufadienolides in Rabbits Plasma
- 中国实验方剂学杂志 2011年17卷第21期页码：135-138
- 作者机构：
- 作者简介：
- 基金信息：
  
  "重大新药创制"科技重大专项(2009ZX09502-008;2009ZX09308-003);教育部博士点基金(20090013110007);国家自然基金课题(81073057)
- DOI：CNKI:11-3495/R.20110906.1107.004
  中图分类号：
- 纸质出版日期：2011
- 稿件说明：
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刘冬, 杜守颖, 何秀峰, 等. 蟾酥提取物中2种蟾蜍甾烯类成分兔体内药代动力学研究[J]. 中国实验方剂学杂志, 2011,17(21):135-138.

LIU Dong, DU Shou-ying, HE Xiu-feng, et al. Studies on Simultaneous Determination and Pharmacokinetics of Two Bufadienolides in Rabbits Plasma[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 135-138.
刘冬, 杜守颖, 何秀峰, 等. 蟾酥提取物中2种蟾蜍甾烯类成分兔体内药代动力学研究[J]. 中国实验方剂学杂志, 2011,17(21):135-138. DOI： CNKI:11-3495/R.20110906.1107.004.

LIU Dong, DU Shou-ying, HE Xiu-feng, et al. Studies on Simultaneous Determination and Pharmacokinetics of Two Bufadienolides in Rabbits Plasma[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 135-138. DOI： CNKI:11-3495/R.20110906.1107.004.

摘要

目的: 建立兔血浆中2种蟾蜍甾烯类化合物蟾毒灵及酯蟾毒配基的分析方法

探讨其在兔体内的药代动力学过程。方法: 兔按0.35 mg·kg-1由耳缘静脉注射蟾酥提取物后分别于2

90 min心脏取血

采用乙腈沉蛋白与液液萃取相结合方法进行血浆样品预处理

以HPLC测定兔血浆中蟾毒灵及酯蟾毒配基的浓度

以Kinetica软件拟合药动学参数。结果: 建立的HPLC方法

蟾毒灵、酯蟾毒配基均得到很好的分离

达到体内分析要求。经非房室模型拟合

得到了蟾毒灵、酯蟾毒配基在兔体内主要药动学参数。结论: 建立的蟾毒灵、酯蟾毒配基血药浓度测定方法及所获得的药动学参数

可为中药蟾酥提取物的药代动力学研究提供参考。

Abstract

Objective: To establish a HPLC method for the assay of bufalin (BL)

resibufogenin (RBG) and study the pharmacokinetics of BL

RBG in rabbits plasma. Method: The sample blood from rabbits were injected venenum bufonis extract by 0.35 mg·kg-1 at 2

90 min. Protein precipitation with acetonitrile and liquid-liquid extraction was applied to purify plasma samples and used HPLC to determine the concentration of BL

RBG in it. The pharmacokinetics parameters were accessed by Kinetica software. Result: In this HPLC method

the separation

precision and accuracy of BL

RBG were well. The calibration curves were linear. The analytical recoveries were all more than 90%

intra and inter-day precision RSD were all less than 15.0% ( n =5). The main pharmacokinetics parameters were fitted by non-compartment models. Conclusion: The HPLC method can be used to determine the concentration and to investigate the pharmacokinetics of BL and RBG.

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