Objective: To illustrate the compatibility of Jinglingizi powder by investigating the influence of Jinglingzi powder with different compatibility on the enzymatic activity of cytochrome P450 3A4(CYP 3A4) from rat liver microsome. Method: The different compatibility of Jinglingizi powder was designed based on the orthogonal array L9(34). In vitro test

rat liver microsome incubation system was applied to detect the 50% inhibitory concentration of Jinglingzi powder with different compatibility. In vivo experiment

rats were administered orally with the different compatibility of Jinglingizi powder (dose: 9.75 g·kg-1) from day 1 to day 5

then injected probe drug testosterone. The biosamples from liver tissue were obtained by microdialysis probe

then to be analysed by HPLC. The concentration of 6-β-testosterone and testosterone were accurately determined. The ratio hepatic concentration of 6-β-testosterone to hepatic concentration of testosterone

was applied to describe the CYP 3A4 enzyme activity. Result: The half maximal inhibitory concentrations (IC50) of the extract of Toosendan fruit

Rhizoma Corydalis and Jinlingzi Powder(formula.1-9) on the enzymatic activity of CYP3A4 were (2.59±0.33)

(0.87±0.30)

(1.14±0.20)

(1.00±0.13)

(1.19±0.10)

(2.33±0.15)

(1.39±0.19)

(1.14±0.20)

(1.29±0.14)

(1.43±0.32)

(1.49±0.28) mg·L-1

respectively. In vivo test

the CYP3A4 enzyme activity was inhibited by the different compatibility of Jinglingizi powder

compared with the normal control group. The inhibited intensity of Jinglingizi powde followed the order: formula 1 (formula 4)> formula 7 > formula 2(formula 5) > formula 8> formula 3> formula 9. Conclusion: The CYP3A4 enzyme activity is inhibited by the different compatibility of Jinglingizi powder

compared with the normal control group. Compatibility of Jinglingizi powder synergetically down-regulate the the CYP3A4 enzyme activity.