双参宁心方的正常与心肌缺血模型大鼠血清对 缺氧/复氧H9C2细胞作用的比较

李丹; 韩笑; 侯金才; 刘建勋

doi:CNKI:11-3495/R.20110920.1429.003

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双参宁心方的正常与心肌缺血模型大鼠血清对缺氧/复氧H9C2细胞作用的比较

更新时间：2024-01-04

- 双参宁心方的正常与心肌缺血模型大鼠血清对缺氧/复氧H9C2细胞作用的比较
- Comparison of Effects of Shuangshen Ningxin Serum Obtained from Normal Rat and Myocardial Ischemia Rat on H/R H9C2 Myocardial Cells
- 中国实验方剂学杂志 2011年17卷第22期页码：127-130
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金重点项目 (30830118);国家科技重大专项"重大新药创制"项目(20092X09303, 2009ZX09301-005-2-11)
- DOI：CNKI:11-3495/R.20110920.1429.003
  中图分类号：
- 纸质出版日期：2011
- 稿件说明：
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李丹, 韩笑, 侯金才, 等. 双参宁心方的正常与心肌缺血模型大鼠血清对缺氧/复氧H9C2细胞作用的比较[J]. 中国实验方剂学杂志, 2011,17(22):127-130.

LI Dan, HAN Xiao, HOU Jin-cai, et al. Comparison of Effects of Shuangshen Ningxin Serum Obtained from Normal Rat and Myocardial Ischemia Rat on H/R H9C2 Myocardial Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 127-130.
李丹, 韩笑, 侯金才, 等. 双参宁心方的正常与心肌缺血模型大鼠血清对缺氧/复氧H9C2细胞作用的比较[J]. 中国实验方剂学杂志, 2011,17(22):127-130. DOI： CNKI:11-3495/R.20110920.1429.003.

LI Dan, HAN Xiao, HOU Jin-cai, et al. Comparison of Effects of Shuangshen Ningxin Serum Obtained from Normal Rat and Myocardial Ischemia Rat on H/R H9C2 Myocardial Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 127-130. DOI： CNKI:11-3495/R.20110920.1429.003.

摘要

目的: 比较大鼠在正常状态与心肌缺血状态下制备的双参宁心方(SSNX)空白及含药血清对体外缺氧/复氧H9C2心肌细胞活力的影响。方法: 分别设立正常组、心肌缺血模型组。大鼠心肌缺血模型由冠状动脉结扎法建立

术后缝合伤口继续喂养。术后2 h除正常空白、模型空白、模型假手术组给予生理盐水外

均按高、中、低剂量(180

45 mg·kg-1)分别ig给予SSNX 5日。末次给药后30

150 min分批处死大鼠

分别制备正常及模型血清。将其作用于缺氧/复氧H9C2细胞。MTT法检测H9C2细胞活力。结果: 正常空白血清与模型空白血清对缺氧/复氧H9C2心肌细胞活力影响差异明显(P<0.05)

正常动物SSNX含药血清与心肌缺血模型动物SSNX含药血清均有提高缺氧/复氧H9C2心肌细胞活力的作用

分别与正常动物空白血清及模型动物血清比较(P<0.05);各时间点正常含药血清与相应时间点模型含药血清相比更显著提高了H9C2心肌细胞活力。结论: 2种方法制备的SSNX血清对体外缺氧/复氧H9C2心肌细胞活力的作用趋势一致

但强度有一定差异。

Abstract

Objective: To compare the effects of pathological model Shuangshen Ningxin (SSNX)serum with normal SSNX serum on hypoxia /reoxygenation (H/R) injury H9C2 cell citality. Method: Model rat of myocardial ischemia injury was established by coronary arterial ligating. Anterior descending branch of coronary artery was ligated. Except for normal blank

model blank

sham operation group

Normal rats and model rats are consecutively gavaged with SSNX at high

medium and low doses 2 h after the operation for 5 days consecutively. Collect blood from abdominal aorta 30

90 and 150 min after last drug administration

and prepare serum. Construct H9C2 myocardial cell H/R injury model. Respectively administrate serums containing drugs from normal rat and model rat to H/R H9C2 cells. Determine the cell citality by using MTT method. Result: There is a difference between normal blank serum group to model blank serum group in cell vitality(P<0.05). Compared to normal blank and model blank group

cell activity SSNX serum group of normal and myocardium ischemia rat model are obviously different (P<0.05); and the effects of SSNX serum group of normal is better than group of model at different time points (P<0.05). Conclusion: The effects of SSNX serum prepared by two ways

is analogous on the role in the trend

but is different on degree.

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