Objective: To sduty the effect of compatibility of Astragali Radix effective parts on granulocyte-macrophage colony-stimulating factor(GM-CSF)

granulocyte colony-stimulating factor (G-CSF)

tumor necrosis factor-β(TNF-β) in myelosuppression mice. Method: BALB/c mice were given cyclophosphamide intraperitoneally at 100 mg·kg-1 on the first

third

fifth day of the experiment to built myelosuppression mice model. The mice were divided into uninfluenced group

model group

Astragali Radix group

ketoside group

glucoside group

saccharon group

glucoside and ketone group. At the same time mice in uninfluenced group and model group were given water ig with the dosage of 10 mL·kg-1. Mice in Astragali Radix group were given the decoction ig with the dosage of 10 mL·kg-1. Mice in groups of compatibility of Astragali Radix effective parts were given corresponding medicine (ig) with the dosage of Astragali Radix 10 g·kg-1 for 15 days: ketoside group 0.227 g·kg-1

glucoside group 0.610 g·kg-1

saccharon group0.514 g·kg-1

glucoside and ketone 0.678 g·kg-1 . Detected GM-CSF

G-CSF

TNF-β

levels in bone marrow cells with ELISA. Result: GM-CSF in glucoside group

saccharon group increased significantly(P<0.05). G-CSF in glucoside group

saccharon group increased significantly and were higher than Astragali Radix group(P<0.05). TNF-β in Groups of compatibility of Astragali Radix decreased significantly and glucoside group was lower than ketoside group

saccharon group

glucoside and ketone(P<0.05). Conclusion: Compatibility of Astragali Radix effective parts may regulate granulocytopoiesis through the regulation of cell factors. Astragali Radix polysaccharides with Astragali Radix flavone or astragaloside accelerated granulocytopoiesis significantly.