Objective: To investigate the influence of Olibanum and Myrrha on the activity of cytochrome P450 and its isoforms. Method: 56 mice were divided into control group and 3 groups treated with Olibanum+ Myrrha(3.15

2.10

1.05 g·kg-1) for 7 days. And other 48 mice were divided into control group and 3 groups that were intragastrically administrated once with Olibanum+Myrrha

Olibanum

Myrrha(3.15 g·kg-1). 30 min after last administration

mice were intraperitoneal injected with pentobarbital sodium

to observe the influence of Olibanum and Myrrha on P450. Forty rats were divided into four groups by random. 3 groups were intragastrically treated with Olibanum+ Myrrha

Olibanum

Myrrha(3.00 g·kg-1) once a day for 14 days and the control group with water. Liver microsomes of rats was prepared to investigate the influence on CYP1A2

CYP2E1 and CYP3A4 of Olibanum and Myrrha by three-drug cocktail approach in vitro. Result: The number of sleeping mice was significantly decreased of groups which were treated with Olibanum and Myrrha(3.15

2.10

1.05 g·kg-1) for 7 days

and yet the number was increased with once administration. The phenacetin and chlorzoxazone metabolic rates of Olibanum and Myrrha and its mixture groups were significantly higher than the control group in liver microsomes

and there was significantly different on dapsone. Conclusion: The activity of CYP1A2 and CYP2E1 was increased with the continuous use of Olibanum and Myrrha

and CYP3A4 was not significantly affected.