Objective: To study relationship between physical and chemical parameters and effect of invigorating Qi and enriching blood of Danggui Buxue decoction

and to explore the feasibility of characterizing the quality of decoction by physical and chemical parameters. Method :Danggui Buxue decoction were prepared by four methods (extracting once with 8 times of water for 1 h; extracting three times with 8 times of water for 2 h; extracting three times with 10 times of water for 1.5 h; extracting three times with 12 times of water for 1 h)

of which physical and chemical parameters such as surface tension

pH value

conductivity and osmotic pressure were determined at 25 ℃. Seventy-seven mice were randomly divided into 7 groups

with 11 in each group.Danggui Buxue decoction groups

E Jiao compound group

Blank group and model group were treated by Danggui Buxue decoction

E Jiao compound

normal saline respectively for eight days. Blood deficiency model mice were made by injecting cyclophosphamide from 5th to 8th day. Temperature and peripheral hemogram of mice were determined at 1.5 h after the last administration. Fifty-five mice were randomly divided into blank group

model group

high-dose of Danggui Buxue decoction group

moderate-dose of Danggui Buxue decoction group and low-dose of Danggui Buxuedecoction group

with 11 in each group

whose effects on temperature and peripheral hemogram of blood deficiency model mice induced by injecting cyclophosphamide were studied too. Finally

relationships among physical and chemical parameters

effect and the content of main active ingredients of Danggui Buxue decoction were studied

and mathematical analysis and regression analysis were used to analyze these data. Result : Through polynomial regression analysis

not only correlation coefficient between physical and chemical parameters and the score of component content and effect

but also correlation coefficient between physical and chemical parameters and the score of effect were more than 0.920 0. It showed that physical and chemical parameters closely correlated with component content and effect of invigorating Qi and enriching blood of Danggui Buxue decoction. Conclusion : Physical and chemical parameters had a good correlation with effect of invigorating Qi and enriching blood of Danggui Buxue decoction. These results preliminarily suggest that such parameters could be used to characterize the quality of decoction.