Objective : To observe biological effects of Tianshengyuan oral solution (TSY)

a new candidate Chinese medicine

in mice with aplastic anemia(AA) induced by benzene and cyclophosphamide. Method : Male CD1 mice(20~24 g) were given cyclophosphamide (50 mg·kg-1

sc

once a day) for 7 days and benzene (2 mL·kg-1

sc

once every one day) for 30 days to develop AA mouse models. Meanwhile

the animals were treated with TSY at different doses (3.1

6.2

12.4 g·kg-1

ig

twice a day) for 30 days. Then peripheral leukocyte(WBC)

erythrocyte(RBC)

hemoglobin (HGB)

hematocrit(HCT)

platelet (PLT)

reticulocyte(RET)

femoral bone marrow nucleus cell(BMNC)

megakaryocyte (MKC)

and its pathological features were examined. Result: The values examined in the model group (except WBC) were remarkably lower than those in the normal group(P<0.05~0.001). TSY(24.8 g·kg-1·d-1)could significantly increase the values of RBC(8.3%

P<0.05)

HGB(8.8%

P<0.05)

HCT(7.4%

P<0.05)

PLT(14.8%

P<0.05)

RET (102.0%

P<0.01)

BMNC(34.7%

P<0.05)

MKC(66.7%

P<0.05) compared with model group. But the increase in WBC was not obvious. Besides

the pathological conditions of femoral bone marrow in more than half of AA mice were better in the group of TSY (24.8 g·kg-1·d-1). Conclusion : TSY could promote the proliferation of hematogenic cells in bone marrow

improve the function of hematopoiesis

and show its biological activities for AA mouse models.