Objective : To explore the regulation of Tianshengyuan oral solution (TSY) for the abnormality of T lymphocyte subsets and the level of hematopoietic cellular factors in mice with immune-mediated aplastic anemia(IAA). Method : T-cell subsets

including CD4+

CD8+

in peripheral plasma were analyzed by flow cytometry. GM-CSF and TNF-α were measured by fluorescence immunoassay. Result : TSY(12.4 g·kg-1·d-1) ig for 14 days could significantly reduce the production of peripheral CD8+

increase in the ratio of CD4+/CD8+(2.34±1.10

n=7

P<0.001) and in the level of GM-CSF·L-1

n=10

P<0.01]

compared with IAA model group·L-1

n=12

respectively]. Meanwhile

the decreased levels of TNF-α were also seen in the same group. Conclusion These results suggest that TSY for the treatment of IAA mice could be by modulating the balance between T-lymphocyte subsets

and the levels of hematopoietic cellular factors.