HUANG Li-ping, CHEN Yao-hui, WU Su-fen, et al. The Effect of Composition of Invigorating Qi and Promoting Blood on Hemorrhagic Blood Deficient Mouse by Qualitative Evenly Design[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 129-134.
HUANG Li-ping, CHEN Yao-hui, WU Su-fen, et al. The Effect of Composition of Invigorating Qi and Promoting Blood on Hemorrhagic Blood Deficient Mouse by Qualitative Evenly Design[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 129-134. DOI: CNKI:11-3495/R.20111107.1623.002.
Objective : To analysis the effects of invigorating Qi and promoting blood on hemorrhagic blood deficient in mouse by using qualitative analysis of uniform design and to find out the optimum prescription composition. Method: Twelve supplement Qi medicines
such as Ginseng Radix Et Rhizoma
Panacis Quinquefolii Radix Et Rhizoma
Codonopsis Radix
Pseudostellariae Radix
Astragali Radix
Atractylodis Macrocephalae Rhizoma
Dioscoreas Rhizoma
Lablab Semen Album
Glycyrrhiae Radix
Jujube Fructus
Acanthopanacisi Senticosi Radix Et Rhizoma Seu Caulis
Ganoderma
and 6 supplement blood medicines
such as Angelicae Sinensis Radix
Rehmanniae Radix
Polygoni Multiflori Radix
Paeoniae Radix Alba
Asini Corii Colla
Longan Arillus
were composed of 24 supplement Qi and engendering blood prescriptions according to U24(242) qualitative analysis of uniform design. The hemorrhagic blood deficient mouse model was obtained with 0.5 mL orbital bleeding. Peripheral blood cells were counted after 1 d
4 d
7 d
10 d modeling to find the best diversify time of potency. The content of RBC
HGB
HCT
WBC
PLT of the obvious effective time and the relationship between effect with administration time were analyzed using principal component analysis method to find out the major cause. Multiple linear regression with dummy variable was set up to find out the best prescription composition. Result: The best diversify time of potency was on 7 d.RBC
HGB
HCT were the major cause indexes. The multiple linear regression were set up and the composition of Ginseng Radix and Astragali Sinensis Radix appears to be the best compatibility for the efficacy. Conclusion : The composition of Ginseng Radix and Astragali sinensis Radix is the best compatibility for blood loss anemia mouse. Qualitative analysis of uniform design is feasible to be used in the compatibility study.