Objective: To investigate the influence of Xiezhuo Chubi decoction on uric acid and urate transporter 1 (URAT1) gene in hyperuricemia mice

that to search for the mechanism of reducing uric acid. Method: Sixty Kunming male mice of SPF grade were randomly divided into high-

medium-

low-dose Xiezhuo Chubi decoction group

Benzbromarone group

model group and control group. All mice were established with yeast method combined with uricase inhibition method to build hyperuricemia model except control group. The corresponding drugs were administrated at the 7th day (Xiezhuo Chubi decoction 37.5

18.75

9.375 g·kg-1·d-1

benzbromarone 20 mg·kg-1·d-1

ig

once per day for 15 days). The blood was obtained to detect uric acid levels. Simultaneously

the kidneys were removed to detect mRNA expressions of URAT1 with fluorescence quantitative PCR. Result: Imposed by different factors to each group

oral administration of Xiezhuo Chubi decoction could significantly reduce the uric acid in mice in a dose-dependent manner (209.00±24.54)

(234.50±31.38)

(273.88±25.04)μmol·L-1 (P<0.05). The effects of high-and medium-dose Xiezhuo Chubi decoction group were similar with benzbromarone group (208.70±33.70) μmol·L-1. The mRNA expressions of URAT1 were decreased significantly in Xiezhuo Chubi decoction group than that in model group (P<0.05). Conclusion: Xiezhuo Chubi decoction could reduce the uric acid effectively. Its mechanism might be the action of inhibition to the mRNA expressions of URAT1 in kidney.