Zhu Qing, Li Guanghui, Hou Xiaoming, et al. Determination of Chlorogenic Acid in Flos Lonicerae and“Liyan Zhike Chongji”by HPLC[J]. Chinese journal of experimental traditional medical formulae, 1996, 2(5): 5-7.
Zhu Qing, Li Guanghui, Hou Xiaoming, et al. Determination of Chlorogenic Acid in Flos Lonicerae and“Liyan Zhike Chongji”by HPLC[J]. Chinese journal of experimental traditional medical formulae, 1996, 2(5): 5-7.DOI:
Chlorogenic acid in Flos lonicerae and“Li Yan Zhi Ke Chong Ji”was determinated by high performance liquid chromatography.Chlorogenic acid was separated on KYWG-C18 column and detected at 323nm
using water-acetonitrile-acetic acid(110:3:3)as mobile phase. There is a good linearity from 0.01 to 0.10 mg/ml of gatrodin(r=0.9998
Y=-2.4605×103+5.6138x105X). The average recovery was 100.87% and the relative standard deviation was 1.55%(n=6).This method is simple