HPLC同时测定冬虫夏草中腺苷、虫草素和麦角甾醇

陈婷; 刘群; 莫灼康; 徐敏嫦; 陈润添

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HPLC同时测定冬虫夏草中腺苷、虫草素和麦角甾醇

更新时间：2024-01-04

- HPLC同时测定冬虫夏草中腺苷、虫草素和麦角甾醇
- Determination of Adenosine, Cordycepin and Ergosterol in by HPLC
- 中国实验方剂学杂志 2013年19卷第6期页码：161-163
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- 纸质出版日期：2013
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陈婷, 刘群, 莫灼康, 等. HPLC同时测定冬虫夏草中腺苷、虫草素和麦角甾醇[J]. 中国实验方剂学杂志, 2013,19(6):161-163.

CHEN Ting, LIU Qun, MO Zhuo-kang, et al. Determination of Adenosine, Cordycepin and Ergosterol in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 161-163.
陈婷, 刘群, 莫灼康, 等. HPLC同时测定冬虫夏草中腺苷、虫草素和麦角甾醇[J]. 中国实验方剂学杂志, 2013,19(6):161-163. DOI：

CHEN Ting, LIU Qun, MO Zhuo-kang, et al. Determination of Adenosine, Cordycepin and Ergosterol in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(6): 161-163. DOI：

摘要

目的: 建立同时测定冬虫夏草药材中腺苷、虫草素和麦角甾醇的方法。方法: Diamosil C18色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-水梯度洗脱

体积流量1.0 mL·min-1

柱温30 ℃

进样量10 μL

检测波长260 nm。结果: 腺苷、虫草素和麦角甾醇分别在0.032 2～0.805

0.015 4～0.385

0.084 3～2.107 5 μg线性关系良好

回归方程分别为Y=2 573X+0.737 8;Y=2 631X+0.624 4;Y=2 315X+0.482 9

平均加样回收率分别为98.94%(RSD 1.16%)

99.73%(RSD 2.19%)

99.55%(RSD 2.02%)。结论: 该方法准确性高

重复性好

可作为冬虫夏草药材的质量控制方法。

Abstract

Objective: To establish the quantitative analysis method for adenosine

cordycepin and ergosterol in Cordyceps sinensis by HPLC. Method: HPLC was performed on Diamosil C18 column(4.6 mm×250 mm

5 μm); the mobile phase consisted of methanol and water at a flow rate of 1.0 mL·min-1.The column temperature was set at 30 ℃

and the eluate was detected at 260 nm. Result: Quantitative analysis of HPLC showed that the linear ranges of adenosine

cordycepin and ergosterol were 0.032 2-0.805

0.015 4-0.385 and 0.084 3-2.107 5 μg respectively.The average recoveries of adenosine

cordycepin and ergosterol were 98.94%(RSD 1.16%)

99.73%(RSD 2.19%)

99.55%(RSD 2.02%)respectively. Conclusion: The method set up is accurate

feasible and reliable

therefore available for the quality control of C. sinensis.

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