Objective: To study the characteristic chromatogram of saponins in effective parts of Xuechangning formula by HPLC. Method: The chromatographic condition of HPLC was as follows: Synergi Fusion-RP column C18(4.6 mm×250 mm

5 μm) at 30 ℃

the mobile phase being acetonitrile with water

gradient elution in chromatographic separation

the detective wavelength being 203 nm

and flow rate being 1.0 mL·min-1. Result: Except for three batches of 12 batches of saponins in Xuechangning formula

the characteristic chromatogram similarity value of the other eight batches was above 0.9 as compared with system generation spectrum

and 19 common characteristic peaks were presented.The results showed that the main chemical constituents of medicinal materials were similar among samples from different habitats

but their relative ratios are significantly different. Conclusion: The method is simple

accurate and reproducibility

and can be used for the quality control of Xuechangning formula.