Objective:To investigate anti-inflammatory and antioxidant function of total flavones of Bidens bipinnata L (TFB) on lipoplysaccharide(LPS)-induced acute lung injury(ALI) and the mechanism. Method: One hundred and twenty SD rats were randomly divided into six groups:control group(physiological saline

ip)

model group

dexamethasone group(5 mg·kg-1

ip)

TFB treatment group(100

150

200 mg·kg-1

ip)

twenty rats in each group. The rat ALI model was induced by LPS

5 mg·kg-1

ip. Six hours after modeling

all rats were sacrificed

protein content

white blood cell in bronchoalveolar lavage fluid(BALF)

expression of NF-κB p65

and level of tumor necrosis factor(TNF-α)

interleukin-6(IL-6)

interleukin-10(IL-10)superoxide dismutase(SOD)

malondialdehyde(MDA) and the activity of myeloperoxidase(MPO) in lung were measured. Result: Compared with control group

content of WBC and the protein in BALF was higher(P＜0.05-P<0.01)

compared with model group

NF-κB p65 expression level was(38.46±4.56)%

(31.39±4.18)% (in TFB high and medium dose group

respectively)

the content of MDA was(1.73±0.19)

(1.46±0.15)nmol·mg-1

and the content of TNF-α was(259.33±37.48)

(211.46±32.69)μg·L-1

and the content of IL-6 was(287.46±100.18)

(223.55±93.49)ng·L-1

and the activitiy of MPO was (2.69±0.57)

(2.43±0.38)U·g-1

respectevly.NF-κB p65 expression level and the content of MDA

TNF-α

IL-6 and the activities of MPO in lung were significantly lower(P＜0.05-P<0.01).On the other hand

the level of IL-10 was(16.73±3.87)

(17.28±3.58)μg·L-1

the content of SOD was (69.46±9.84)

(73.24±8.39)U·mg-1

the content of IL-10 and SOD was increased significantly(P＜0.05-P<0.01). Conclusion: TFB can reduce pulmonary vascular permeability of LPS induced acute lung injury

reduce the inflammatory exudation and oxidative stress damage.