YI Wei-dan, FANG Ying-quan. Determination of saikosaponin a of var. from the Three Gorges Reservoir Area by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 71-73.
YI Wei-dan, FANG Ying-quan. Determination of saikosaponin a of var. from the Three Gorges Reservoir Area by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 71-73.DOI:
Objective: To establish a method for the determination saikosaponin a from Bupleurum longiradiatum var. porphyranthum. Method: HPLC conditions: Phenomenex luna C18 column (4.6 mm×250 mm
5 μm) with methanol-water (74: 26) as mobile phase; flow rate was 0.9 mL· min-1; column temperature was set at 35℃
and the detection wavelength was at 200 nm. Result: Saikosaponin a was linear in the range of 0.11-4.4 μg(r=0.999 9).The average recovery was 97.42% with RSD of 1.42%. Conclusion: HPLC gradient elution method for determination of saikosaponin a from B. longiradiatum var. porphyranthum is sensitive