Objective:To explore the effect of the flavonoids of Ilex pubescens on blood stasis with cerebral ischemia in rats. Method: Dexamethasone was used to induce blodd stasis model. Suspension of flavonoids of I. pubescens was given for 10 days

the carotid artery was blocked for 30 min 1 h after administration of determining on the day 11.Viscosity of whole blood was measured and brain tissue was collected for HE staining and determination of lactic acid(LD)

lactate dehydrogenase(LDH)

adenosine triphosphate(ATP) enzyme levels and malondialdehyde(MDA) content. Result: The model of blood stasis with cerebral ischemia was successfully induced. Compared with model group the large and medium doses of the flavonoids of I. pubescens could reduce the viscosity of whole blood significantly

the value was (7.25±1.78)

(7.52±1.06)

(8.75±2.02)

(9.26±1.35)

(17.06±3.77)

(18.36±2.29)mPa·s. The small dose of the flavonoids of I.pubescens could reduce the whole blood viscosity

value being (7.92±2.32)mPa·s. The medium dose of the flavonoids of I. pubescens could reduce the LD content(3.51±0.69)mmol·g-1

increased LDH level(1 822.2±128.0)U·mg-1 significantly and lower MDA content (2.66±0.42)nmol·mg-1

The large and medium dose of the flavonoids of I.pubescens could significantly improve the Na+-K+-ATPase

Mg2+-ATPase

Ca2+-Mg2+-ATP activity

values were (8.230±0.633)

(8.53±0.52)

(7.15±0.20)

(7.98±0.24)

(8.17±0.33)

(8.54±0.29)μmol·mg-1·h-1. The medium dose of the flavonoids of I. pubescens could significantly improve the Ca2+-ATPase activity(8.84±1.00)μmolpi·mg-1·h-1. The small dose of the flavonoids of I.pubescens could significantly improve the Na+-K+-ATPase activity(7.94±0.16)μmol·mg-1·h-1. Each group of the flavonoids of I.pubescens could significant improvement the pathological changes in brain tissue. Conclusion: The flavonoids of I.pubescens can improve the cerebral ischemia injury of the blood stasis with cerebral ischemia.