Objective: To determine and compare the content of myo-inositol in Citri Grandis. Method: The content was determined by high performance liquid chromatography coupled with evaporative light-scattering detector (HPLC-ELSD) method. The operation was performed on a Rezex RCM-Monosaccharide Ca2+ (8%) cloumn (7.8 mm×300 mm) using deionized water as the mobile phase at the flow rate of 0.4 mL· min-1. The temperature of drift tube was set at 80℃

the tube was set at heating 100% and the column temperature at 35℃

the gas pressure was 40 bar. Result: The calibration curve of inositol displayed ideal linearity over the range of 0.25-8.00 g· L-1 (r=0.999 9)

and the average recovery was 98.94% with RSD of 2.19%. The other ingredients in the sample showed no interference. Conclusion: The content of myo-inositol was different due to the different sample-variety of C. grandis and the sample from Lufu was the highest. And it would show a pattern of increase after pollination. But this study didn't show variations for samples from different region.