Objective: To observe the effects of Yinchenhao Decoction(YCHD) on non-alcoholic steatohepatitis (NASH) rats induced by high fat diet. Method: NASH model in SD rats was established by feeding high fat diet for 8 weeks

and then the rats were randomized into model group

YCHD high dose group

YCHD low dose group

composite prescription Yiganling group

natural recovery group. Rats in medication groups were administrated ig with corresponding herbs from the 9th week

YCHD groups (4.97

3.31 g·kg-1)

prescription Yiganling group (1.265×10-2 g·kg-1) and natural recovery group was given normal diet.After treatment for 4 weeks

the influence of the treatments on blood lipids

liver lipids

antioxidant indexes and serum insulin contents were investigated. Hepatic tissues were detected by HE and oil red staining. Result: The level of total cholesterol(TC)

triglycerides(TG)

low density lipoprotein(LDL-C)

malondialdehyde(MDA)

insulin(INS)

free fatty acids(FFA)

MDA in liver of YCHD low dose group were significantly lower than those of model group. The activities of glutathine peroxidase (GSH-Px) was significantly higher than that of model group(P<0.05

P<0.01). It can improve the steatosis of liver in rats with NASH.Meanwhile

the level of TG

Ins

MDA in serum and FFA in liver of YCHD high dose group were also significantly lower than those of model group(P<0.05

P<0.01). Conclusion: YCHD low dose is better than high dose on rats NASH model. YCHD low dose has good effects on NASH rats feeding by high fat diet with disorders of lipid metabolism and hyperinsulinemia for the pathogenesis of NAFLD ‘two-hit’ mechanism by enhancing antioxidant ability and clearing free radical metabolites as well as inhibiting lipid peroxidation.