高效毛细管电泳法测定加味逍遥丸中芍药苷、栀子苷的含量

陈玉杰

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高效毛细管电泳法测定加味逍遥丸中芍药苷、栀子苷的含量

更新时间：2024-01-04

- 高效毛细管电泳法测定加味逍遥丸中芍药苷、栀子苷的含量
- Determination of Paeoniflorin and Geniposide in Jiawei Xiaoyao Pills by High Performance Capillary Electrophoresis
- 中国实验方剂学杂志 2012年18卷第10期页码：146-149
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- 纸质出版日期：2012
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陈玉杰. 高效毛细管电泳法测定加味逍遥丸中芍药苷、栀子苷的含量[J]. 中国实验方剂学杂志, 2012,18(10):146-149.

CHEN Yu-jie. Determination of Paeoniflorin and Geniposide in Jiawei Xiaoyao Pills by High Performance Capillary Electrophoresis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 146-149.
陈玉杰. 高效毛细管电泳法测定加味逍遥丸中芍药苷、栀子苷的含量[J]. 中国实验方剂学杂志, 2012,18(10):146-149. DOI：

CHEN Yu-jie. Determination of Paeoniflorin and Geniposide in Jiawei Xiaoyao Pills by High Performance Capillary Electrophoresis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 146-149. DOI：

摘要

目的: 建立加味逍遥丸中芍药苷、栀子苷同时测定的高效毛细管电泳分析方法。方法: 采用高效毛细管电泳法

缓冲体系为25 mmol·L-1硼砂缓冲液(NaOH调pH 10.5)

熔融石英毛细管(60 cm×50 μm)

运行电压25 kV

柱温25 ℃

检测波长230 nm。结果: 芍药苷、栀子苷分别在0.008 1~0.162 6 g·L-1和0.023 8~0.475 6 g·L-1线性关系良好(r=0.999 8和0.999 7)

样品平均加样回收率为99.67%(RSD 1.27%)和99.36%(RSD 1.53%) 结论: 该方法简单、快速准确、重复性好

适用于加味逍遥丸的质量控制。

Abstract

Objective: To establish a new high performance capillary electrophoresis(HPCE) method for determination of paeoniflorin and geniposide in Jiawei Xiaoyao pills. Method: Paeoniflorin and geniposide in Jiawei Xiaoyao pill were determined by using HPCE. The background electrolyte system selected was 25 mmol·L-1 sodium borate buffer(pH was adjusted to 10.5 by caustic soda). Capillary electrophoresis was performed using 60 cm×50 μm fused silica capillary column

applied voltage was 25 kV; temperature was 25 ℃. The detection wavelength was set at 230 nm. Result: The linear range of paeoniflorin and geniposide was 0.008 1-0.162 6 g·L-1 and 0.023 8-0.475 6 g·L-1(r=0.999 8 and 0.999 7)

and the average recovery was 99.67%(RSD 1.27%)and 99.36%(RSD 1.53%). Conclusion: The method is simple

rapid and reproducible

and could be used for the quality control of Jiawei Xiaoyao pills.

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