YI Hang, HE Chun-bin, PENG Chang-chun, et al. Optimization of Continuous Countercurrent Extraction Process of Egui Yangxue Prescription[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 52-54.
YI Hang, HE Chun-bin, PENG Chang-chun, et al. Optimization of Continuous Countercurrent Extraction Process of Egui Yangxue Prescription[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 52-54.DOI:
Objective: To optimize continuous countercurrent extraction process of Egui Yangxue prescription. Method: With the content of ferulic acid and the amount of extract as indexes
the content of ferulic acid was determined by HPLC
optimum continuous countercurrent extraction process of Egui Yangxue prescription was determined by multi-index comprehensive evaluation method and orthogonal design. Factors of extraction time
solid-liquid ratio
the concentration of ethanol and extraction temperature were investigated. Result: Optimum extraction process was:solid-liquid ratio of 1 : 16 (equivalent to each level liquid ratio of 1 : 4)
extracted 20 min with 60% ethanol under extraction temperature of 40 ℃. Conclusion: This optimized extraction process was stable and reliable
and it could provide scientific basis for relative research of Egui Yangxue prescription.