Objective: To optimize extraction technology of total tritepenoidic acid from Fomes officinalis and establish its content determination method. Method: The content of total tritepenoidic acid was determined by UV-Vis with oleanic acid as reference;Effects of kind and concentration of extraction solvent

solid-liquid ratio

temperature

extraction time and extraction times on yield of total tritepenoidic acid were investigated by single factor test

basis on this

extraction technology was optimized by orthogonal test. Result: Optimum conditions of ultrasonics extraction were as follow:extracted 3 times at 60 ℃ with 30 times the amount of 90% ethanol

30 min each time.Under these conditions

effect of factors on yield was in order of extraction temperature＞solid-liquid ratio＞extraction time＞the concentration of ethanol

yield of total tritepenoidic acid from F. Officinalis was 35.1%. Conclusion: The content of triterpenoidic acid from F. officinalis was very high

this optimized technology was stable and feasible

which could be extended to large-scale production.