Objective: The content distribution of total flavonoids in different parts of Berchemia lineate from city of DuYun in Guizhou was investigated. Method :Flavonids in different parts of Berchemia Lineate(L)DC. were extracted by ethanol as solvent reflux

The total flavonoids were determined by spectrophotometry

rutin as control sample. Result: The absorbency and concentration of rutin have good linear correlation in the range of 5-30 μg·L-1.The regression equation was Y=0.011 2X+0.041 9(r=0.999 1)

the average recovery rate was 98.56%

Accuracy experiment RSD 0.279%

repeatability test RSD 0.634%

stability test RSD 1.49%

the contents of total flavonoids in leaf and stem was respectively 12.30% and 1.42%

that the means content of total flavonoids in leaf is 8.7 times higher than that in stem. Conclusion: the content of total flavonoids in the leaf and in the stem has a big difference

thereby the leaf of B. lineate from Duyun in Guizhou has value to be developed and used.