Objective: To improve the quality standard of Flos Sophorae Tostus in Chinese Pharmacopoeia (2010)

according to the limitation of the content of total flavonoids

rutin

quercetin in the processed pieces. Method: The content of total flavonoids was determined by UV-Vis Spectrophotometry. The content of rutin and quercetin were determined by HPLC. Octadecylsilane bonded silica gel was used as filling material. Mobile phase for rutin consisted of methanol-1% glacial acetic acid (32: 68). Mobile phase for quercetin was methanol-water-glacial acetic acid(44: 56: 0.4). The detection wavelengths were all at 257 nm. Result: The content of total flavonoids

rutin

quercetin was no lower than 8%

6%

0.35% in flower and no lower than 20%

16%

0.3% in flower buds. Conclusion: These methods are simple

accurate and with good reproducibility. The established quality standards improve the quality control and provide experimental datas for safety and efficacy of clinical application of Flos Sophorae Tostus.