Objective: The present study is designed to observe the protective effects of baicalein against hepatotoxins-induced cytotoxicity on the human normal liver L-02 cells. Method: L-02 cells were seeded and pretreated with or without baicalein (1

10

25

50

100 μmol·L-1) for 15 min

and then cells were further treated with or without various hepatotoxins

including acetaminophen (AP

10 mmol·L-1)

clivorine (CLI

50 μmol·L-1)

toosendanin (TSN

2 μmol·L-1) and ethanol (EtOH

100 μmol·L-1) for 48 h. The survival cells were determined by MTT assay. Result: Four hepatotoxins could significantly reduce the cell viability (P< 0.001) compared with the control group. After treatments

baicalein could increase the cell viability which was decreased by AP

TSN and EtOH (P<0.01

P<0.001). Moreover

baicalein could significantly increase the cell viability which was decreased by CLI (P<0.05

P<0.001). Conclusion: Baicalein can prevent the cytotoxicity induced by those hepatotoxins in human normal liver L-02 cells.