Objective: To resarch chemical composition change after enrichment and purification of alkaloids from Aconitum carmichaelii with D101 macroporous resin. Method: Taking transfer rate of total alkaloids

diester diterpenoid alkaloids

aconitine

hypaconitine and mesaconitine as indexes

before and after purification

the content of alkaloids from extracts of A. carmichaelii was determined by UV and HPLC

ingredients of alkaloids differentiated by TLC. Result: Alkaloids from A. carmichaelii was enriched by D101 macroporous resin

transfer rate and purity of total alkaloids were 83.70%

67.34%

respectively;Transfer rate of aconitine

hypaconitine and mesaconitine were 77.78%

94.12%

52.63%;It showed 6 similar biological spots by TLC comparison

this indicated that there was no significant difference before and after enrichment of alkaloids composition. Conclusion: D101 type macroporous resin could effectively enhance purity of total alkaloids from A. carmichaelii with high transfer rate of alkaloids

it could be used for production promotion.