Objective: To measure the content of baicalin in root

stem and leave of Scutellaria baicalensis growing in different habitat. Method: The content of baicalin was determined by HPLC. The chromatographic condition included Kromasil ODS (4.6 mm×250 mm

5 μm) and mobile phase consisting of a mixture of methanol-H2O (53: 47). Baicalin was detected at 280 nm. The standard curve was linear in the range of 0.124-0.93 μg with correlation coefficient 0.999 9. The average recovery of baicalin was 98.17% with RSD 1.3% (n=6). Result: The average content of baicalin in different habitat root was 6.97%

6.01%

5.49%

3.50% and 4.38% respectively. In different habitat stem

the average content of baicalin was 0.10%

0.08%

0.09%

0.06% and 0.03% respectively. In different habitat leave

the average content of baicalin was 0.27%

0.26%

0.15%

0.14% and 0.09% respectively. Conclusion: The method of distill is simple and stable. The determination method was quick with good reappearance. Produced in the same area

the content of baicalin of S. baicalensis in different habitat and different organ had significant different.