MENG Jian-sheng, JIANG Jun-chun, ZHOU Xiao. HPLC Determination of Kaempferol in Zhixionghuoxie Pills[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 109-110.
MENG Jian-sheng, JIANG Jun-chun, ZHOU Xiao. HPLC Determination of Kaempferol in Zhixionghuoxie Pills[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 109-110.DOI:
Objective: To establish an HPLC method for the determination of kaempferol in Zhixionghuoxie Pills. Method: The chromatographic system was consisted of C18 column with methanol-water (52 ∶48) as mobile phase. The content was detected at a waveleng of 367 nm; the flow rate was 1.0 mL·min-1. Result: The kaempferol was linear in the range of 2.148-21.48 μg·min-1 (r=0.999 9); the average recovery was 99.08% and RSD 0.47% (n=6). Conolusion: The established method is accuracy
sensitive and simple. It can be used for quality control of Zhixionghuoxie Pills.