Objective: To observe the effect of combined Oldenlandia diffusa and Scutellariae Barbatae Herba superfine powder on tumor growth of transplanted hepatocarcinoma in mice and expressions of Bcl-2 and Bax proteins in tumor tissues. Method: Sixty healthy Kunming mice

transplanted by H22 hepatocarcinoma cells

were divided into 6 groups

including model group

Ruanjian oral liquid group

combined O. diffusa and Scutellariae Barbatae Herba common grinding powder group and three groups of combined O. diffusa and Scutellariae Barbatae Herba superfine powder in high dose

medium dose and low dose.The mice were executed after ten days of treatment and tumor tissues were excised.The size of tumor tissues were measured and tumor inhibition rate was calculated.And expression of Bcl-2 and Bax was detected by using immunohistochemical method. Result: The tumor inhibition rate of Combined O. diffusa and Scutellariae Barbatae Herba superfine powder group in high dose was 47.55%

which was nearly same as Ruanjian Oral Liquid group.Compared with the model group

expression of Bcl-2 protein was decreased in the groups of Combined O. diffusa and Scutellariae Barbatae Herba superfine powder in high

medium and low dose; expression of Bax protein was increased in the groups of combined O.diffusa and Scutellariae Barbatae Herba superfine powder in high and medium dose. Compared with common grinding powder groupvexpression of Bcl-2 protein was increased and the expression of Bax Protein was decreased in the high group of Combined O.diffusa and Scutellariae Barbatae Herba superfine powder. Conclusion: Combined O. diffusa and Scutellariae Barbatae Herba superfine powder can inhibit the growth of tumor of H22

reduce the expression of Bcl-2 protein and increase the expression of Bax

which is worth further studying in antitumor action.