Objective: To establish a method for the determination of reference substances in salvianolic acid A. Method: Diamonsil C18 column(4.6 mm×250 mm

5 μm)

acetonitrile-0.2% phosphoric acid solution(30 ∶70)as mobile phase

flow rate 1 mL·min-1

detection wavelength 285 nm

column temperature 30 ℃

other relative substances were controlled by main component self-compare method. Result: The content of salvianolic acid C was less than 1.0%

and the others were less than 3.0%. Conclusion: This method is specific

sensitive

simple

accurate

and can effectively detect salvianolic acid C.