Objective: To establish a HPLC method for determination of geniposide in Biyuanshu granules. Method: The Kromasil C18 column(4.6 mm×150 mm

5 μm)was used

and mobile phase was composed of CH3OH-0.2% H3PO4(30 ∶70). Detection wavelength was at 238 nm

the flow rate was 1.0 mL·min-1

column temperature was at 35 ℃. Result: The linear response range was from 0.04-0.51 μg(r=0.999 9).The recovery rate was 100.35%(n=5)

RSD was 1.31%. Conclusion: The HPLC method is simple

accurate

and suitable for the determination of geniposide in Biyuanshu granules.