Objective: To establish fingerprint of Fuyankang enema by HPLC-DAD

and to analyze its chemical component classification. Method: The samples were separated on a reversed phase Kromasil C18 column (4.6 mm×250 mm

5 μm) with gradient elution. The mobile phase consisted of acetonitrile-0.04 mol·L-1 potassium dihydrogen phosphate solution containing 0.1% phosphoric acid

the detection wavelength was 230 nm

the flow rate was 1.0 mL·min-1 and column temperature was set at 30 ℃. Result: Thirteen characteristic peaks were obtained in the fingerprint of Fuyankang enema with good separation. The method was stable with great reproducibility. The sources of characteristic peaks in Fuyankang enema were determined with the information of chemicals of compound

negative samples and single medicine. Conclusion: The fingerprint of Fuyankang enema showed a strong characteristic and good reproducibility

and was an important reference for quality evaluation of Fuyankang enema.