反相高效液相色谱测定腰痛宁贴膏中士的宁及马钱子碱含量

秦婷; 李平; 郑艳春; 王春民; 张瑞华

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反相高效液相色谱测定腰痛宁贴膏中士的宁及马钱子碱含量

更新时间：2024-01-04

- 反相高效液相色谱测定腰痛宁贴膏中士的宁及马钱子碱含量
- Determination of Brucine and Strychnine in Yaotongning Patch by RP-HPLC
- 中国实验方剂学杂志 2011年17卷第17期页码：78-80
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- 纸质出版日期：2011
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秦婷, 李平, 郑艳春, 等. 反相高效液相色谱测定腰痛宁贴膏中士的宁及马钱子碱含量[J]. 中国实验方剂学杂志, 2011,17(17):78-80.

QIN Ting, Li Ping, ZHENG Yan-chun, et al. Determination of Brucine and Strychnine in Yaotongning Patch by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 78-80.
秦婷, 李平, 郑艳春, 等. 反相高效液相色谱测定腰痛宁贴膏中士的宁及马钱子碱含量[J]. 中国实验方剂学杂志, 2011,17(17):78-80. DOI：

QIN Ting, Li Ping, ZHENG Yan-chun, et al. Determination of Brucine and Strychnine in Yaotongning Patch by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 78-80. DOI：

摘要

目的: 建立反相高效液相色谱法同时测定腰痛宁贴膏中士的宁和马钱子碱含量。方法: 色谱柱Intersil C8-3(4.6 mm×250 mm

5 μm)

流动相甲醇-水-冰乙酸(20 ∶96 ∶7

用三乙胺调pH为3.10)

流速1.0 mL · min-1

检测波长254 nm

柱温30 ℃。结果: 士的宁在0.020 6~0.123 4 μg线性关系良好(r=0.999 8)

测得平均加样回收率(n=9)为100.28%

RSD 1.33%;马钱子碱在0.013 8~0.083 0 μg线性关系良好

r=0.999 9

测得平均加样回收率(n=9)为99.82%

RSD 0.84%。结论: 方法灵敏、准确、可用于腰痛宁贴膏中士的宁和马钱子碱的质量控制。

Abstract

Objective: To establish the RP-HPLC method for the content determination of brucine and strychnine in Yaotongning Patch. Method: The Intersil C8-3 (4.6 mm×250 mm

5 μm) column was used; the mobile phase consisted of methanol-water-acetic acid (20 ∶96 ∶7)

adjust pH to 3.10 with triethylamine. The flow rate was 1.0 mL ·min-1 and the detection wavelength was at 254 nm; the column temperature was at 30 ℃. Result: The linear range of strychnine was between 0.020 56-0.123 36 μg (r=0.999 8)

the average recovery was 100.28% with a RSD of 1.33% (n=9); the linear range of brucine was between 0.013 84-0.083 04 μg (r=0.999 9); the average recovery was 99.82% with a RSD of 0.84% (n=9). Conclusion: The method is fast

reliable and accurate

it can be used in the quality control of strychnine and brucine in Yaotongning Patch.

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