Objective: To study the effect of Yanshuai mixture (YSM) on the spleen tissue structure and serum interleukin 2 (IL-2) levels in D-galactose induced sub-acute aging mice. Method: The aging mice were established by D-galactose (120 mg·kg-1·d-1) and administered continuously with YSM for 8 weeks. The spleen index and changes in spleen endoscopic biopsy morphology were observed

IL-2 levels in serum were detected using enzyme-linked immunosorbent assay (ELISA). Result: The spleen index of aging model mice was decreased (0.25±0.05)%

histopathological changes in spleen were degraded significantly

and IL-2 level (0.90±0.17)μg·L-1 was significantly reduced compared with those in normal control group (P<0.05). After the treatment with YSM

spleen histomorphological changes were ameliorated

the spleen index of high-dose YSM mice(0.37±0.04) was increased and level of IL-2 in the serum (2.47±0.77)μg·L-1 was increased (P<0.05). High-dose YSM group was superior to Bushen Yishou capsule (BSYS) group

low and middle-dose YSM group (P<0.05). Conclusion: YSM can improve the spleen histopathological changes

inhibit the decline of IL-2 level in serum

and improve immune function

so it may have a good anti-aging effect.