ZHOU Cheng-yan, MA Hong-cui, WANG Mei, et al. Determination and Comparison of Content of Total Flavonoids and TotalPolysaccharides in Leaves and Fruit of during Different Harvest Periods[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 111-114.
ZHOU Cheng-yan, MA Hong-cui, WANG Mei, et al. Determination and Comparison of Content of Total Flavonoids and TotalPolysaccharides in Leaves and Fruit of during Different Harvest Periods[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 111-114.DOI:
Objective: To make clear the peak content of total flavonoids and total polysaccharide in the leaf and fruit of Eucommia ulmoides
so as to provide evidence for the optimal harvest of the fruit of E.ulmoides. Method: Total flavonoids extracted with 70% ethanol by reflux from leaves and fruits of E.ulmoides in different harvest periods were determined by UV spectroscopy at 506 nm. Total polysaccharide from leaves and fruits of E. ulmoides in different harvest periods were determined by phenol-sulfuric acid method and measured by ultraviolet spectrophotometer at 481 nm with glucose as a reference substance. Result: The flavonoids content in leaves and fruits of E. ulmoides during different harvest periods were significant different by UV spectroscopy. The highest flavonoids content of leaves and fruits during different harvest periods of E. ulmoides was found in April
and then declined until July and a little rise again. The content of flavonoids stabilized after September. There were significant differences about total polysaccharide in leaves and fruit of E. ulmoides in different seasons. The total polysaccharide content of the leaves and fruits of Eucommia in different harvests were highest in March. The content of polysaccharide of the Eucommia leaf gradually decreased after May. And then
total polysaccharide content of Eucommia fruits would stabilize from August to November. Conclusion: It can be obtained from this experiment that the best harvest period of the leaves and fruits of Eucommia is in April.