Objective: To investigate astragaloside and astragalan determination method in angelicae sinensis decoction for supplementing blood granules. Method: This article used HPLC to determine the content of astragaloside

and used phenol-sulfuric colorimetric method to determine the content of astragalan. Result: The average recoveries of astragaloside and astragalan were 97.64%

97.68%. Relative standard deviation was 2.10%

3.07%. Conclusion: HPLC had been used in determining the content of astragaloside and it could get an effective separation

good peak shape and reproducible assay results. Phenol-sulfuric colorimetric method was used to determine the content of astragalan

and it was accurate

reliable

high sensitivity

and good reproducibility. So they can be effectively used to evaluate the quality of angelicae sinensis decoction for supplementing blood granules.