银黄煎液多级膜分离物有效成分的含量及其体外抑菌作用

杨丽平; 孔焕宇; 朱嘉; 宋庆慧; 郭武艳

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银黄煎液多级膜分离物有效成分的含量及其体外抑菌作用

更新时间：2024-01-04

- 银黄煎液多级膜分离物有效成分的含量及其体外抑菌作用
- Research of Three Effective Composition and Effect of Bacteriostasis of Yinhuang Decoction by Multi-stage Membrane Filter
- 中国实验方剂学杂志 2011年17卷第11期页码：5-7
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- 基金信息：
  
  国家自然科学基金课题(30873456)
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- 纸质出版日期：2011
- 稿件说明：
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杨丽平, 孔焕宇, 朱嘉, 等. 银黄煎液多级膜分离物有效成分的含量及其体外抑菌作用[J]. 中国实验方剂学杂志, 2011,17(11):5-7.

YANG Li-ping, KONG Huan-yu, ZHU Jia, et al. Research of Three Effective Composition and Effect of Bacteriostasis of Yinhuang Decoction by Multi-stage Membrane Filter[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 5-7.
杨丽平, 孔焕宇, 朱嘉, 等. 银黄煎液多级膜分离物有效成分的含量及其体外抑菌作用[J]. 中国实验方剂学杂志, 2011,17(11):5-7. DOI：

YANG Li-ping, KONG Huan-yu, ZHU Jia, et al. Research of Three Effective Composition and Effect of Bacteriostasis of Yinhuang Decoction by Multi-stage Membrane Filter[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 5-7. DOI：

摘要

目的: 研究银黄煎液及其五级膜截留液中黄芩苷等3个有效成分的含量变化和体外抑茵作用。方法: 采用多级膜分离方法按相对分子质量大小分离银黄煎液

HPLC测定成分含量

用金黄色葡萄球菌和肺炎链球菌体外测试原液及各级分离物的最低抑菌浓度(MIC)。结果: 抑菌作用由强到弱依次为4级、3级、5级、2级、原液、1级。结论: 膜分离方法可分段分离抑菌活性物质;微滤后的银黄煎液各级膜分离物抑菌作用均比原液强。

Abstract

Objective: To research Yinhuang decoction and its five stage membrane filter liquor

to analyze the content change of baicalin and other two kinds effective composition and in vitro effect of bacteriostasis. Method: Multi-stage membrane separation by molecular size was used to separate Yinhuang decoction. Ingredients were determined by HPLC. Staphylococcus aureus and Streptococcus pneumoniae were used to test the minimum inhibitory concentration (MIC) of source liquid and all levels filter liquor. Result: In vitro effect of bacteriostasis from high to low order was four

three

five

two

source

one level filter liquor. Conclusion: Membrane separation method can be used to isolate antimicrobial activity substances by section. The antibacterial effect of membrane filter liquor after microfiltration is stronger than source decoction.

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