Objective: To analyze the fatty acid and other fat-soluble components in seeds of Semen Plantaginis. Method: The seeds of Semen Plantaginis was smashed and screening by 40 of mesh sieve

and extracted by using petroleum ether (60~90 ℃).The extractionwas concentrated and deal by the methylated method. After that

the fatty acids and other-fat-soluble components were determined by GC-MS

and used area normalization method for quantitative analysis. Result: 50 substances were detected from the petroleum etherextraction of seeds of Semen Plantaginis that identified 42 compounds

25 kinds of fatty acids and 17 non-fatty acids. Conclusion: The major fatty acids are 9

12

15-(Z

Z

Z)-octadecatrienoic acid(30.09%)

9

12-(Z

Z)-octad-ecadienoie acid(25.91%)

13-(Z)-docosenoic acid(7.60%)

14-methyl-pentadecanoic acid(7.41%) as well as the rare fatty acids of 2-hexyl-cyclopropaned-ecanoic acid(6.51%)

2-octyl-cyclopropaneoctanoic-acid(0.60%)and 2- cyclopropyl]methyl]-cyclopropaneoctanoic acid(0.07%).