Objective: To investigate the effect of Scutellaria barbata extract for survivin protein expression in human lung cancer A549 cell and its mechanism. Method :Using the MTT assay to display the sensitivity to the drug

the inhibition of S.barbata extract to lung cancer cell(A549

10% fetal calf serum

1640 nutrient medium)in vitro was observed. TUNEL was used to test the apoptosis

immunocytochemistry was applied to examine the expression of survivin protein. Result: The inhibition rate of S.barbata extract(5~40 μg ·mL-1)to A549 indicated a dose-dependent anti-proliferative effect by MTT assay(P＜0.01).The apoptotic index was(38.40±7.41)% in experiment group(S.barbata extract 18.86 μg ·mL-1)

higher than that(6.22±0.45)%in control group

showed a significant difference(P＜0.01). Compared with control group

the masculine cells of survivin protein was decreased in the experiment group obviously(P＜0.01). Conclusion: The proliferation of A549 cell was inhibited obviously by S. barbata extract and the mechanism maybe related with survivin expression downregulation.