Objective: To establish a method for determination of berberine hydrochloride

phellodendrine and timosaponin BⅡ in common anemarrhena rhizome and amur corktree bark extraction. Method: HPLC-UV-ELSD was used in quantitative analysis with condition as follows: Dikma C18(4.6 mm×250 mm

5 μm) was used in analysis; mobile phase was acetonitrile-0.2% acetic acid

gradient elution; flow rate was 1.0 mL ·min-1; column temperature was at 30 ℃. Both UV and ELSD were used as detector. UV wavelength of 280 nm; evaporative light scattering detector drift tube temperature was at 46 ℃; nitrogen pressure: 1.5 Bar. Result: The linear ranges of berberine hydrochloride

phellodendrine and timosaponin BⅡ were 1.008-3.528 μg(r=0.999 5)

0.322-1.127 μg(r=0.999 6)

0.702-2.457 μg(r=0.999 8)

the average recoveries were 100.11%

98.97%

99.56% with the RSD 1.08%

0.90%

1.37%. Conclusion: This quantitative determination method is simple

well reproducible

accurate and reliable.