Objective: To establish the method for determination of berberine hydrochloride and chlorogenic acid in Zhizi jinhuawan by capillary electrophoresis. Method: The separation was performed on a fused silica capillary of 60 cm×75 μm ID (52 cm of effective length). 0.2 moL ·L-1 NaH2PO4 +CH3CH2OH(50 ∶50)(pH 5.50)and 50 mmol ·L-1 Na2B4O7 were selected as the running buffers. The separation voltages were 21 kV and 12 kV

gravity injection (10 s

15 cm). The detection wavelengths were 265 nm and 327 nm. Result: The concentration ranges of determination for berberine hydrochloride and chlorogenic acid were 11.0-44.0 mg ·L-1 and 3.6-14.4 mg ·L-1 with linear ralationship ( r = 0.999 3). The average recoveries were 100.0% and 98.1%

precisions of the method were 1.72% and 0.96%(RSD

n = 6). The average content of berberine hydrochloride was 3.39 mg ·g-1

chlorogenic acid was 0.88 mg ·g-1. Conclusion: The method was accurate

sensitive and rapid for the quality control of Zhizi Jinhuawan.