Objective: To establish a method for the determination of mangiferin and timosaponin BⅡin Anemarrhenae Rhizoma from different areas. Method: The mobile phase for the determination of mangiferin consisted of acetonitrile-0.85% phosphoric acid (12 ∶88)

with the flow rate of 1.0 mL ·min-1

at 35 ℃. The detection wave length of magiferin was set at 258 nm;And the mobile phase for the determination of timosaponin BⅡconsisted of acetonitrile-water (25 ∶75)

with the same flow rate of 1.0 mL ·min-1

at 25 ℃. An evaporative light-scattering detector (ELSD) was used as detector to determine timosaponin BⅡwith the drift tube temperature at 105 ℃. Result: The content of mangiferin and timosaponin BⅡ in Anemarrhenae Rhizoma from different areas were 0.99%~1.68% and 3.90%~10.25% respectively. Conclusion: The method for determination of mangiferin and timosaponin BⅡhere proved to be accurate and suitable

which can be used for the quality control of Anemarrhenae Rhizoma from different areas.