GE Liang, ZHANG Hao-ke, TIAN Shu-ge. Content Determination of Arctiin and Arctigen from Different Parts of Arctium tomentosum by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 41-43.
GE Liang, ZHANG Hao-ke, TIAN Shu-ge. Content Determination of Arctiin and Arctigen from Different Parts of Arctium tomentosum by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 41-43.DOI:
Objective: To establish an HPLC method for determination of different parts of Arctium tomentosum . Method: Chromatographic separation was achieved by using RP-HPLC system consisting of a Shimadzu LC-6AD and Kromasil C18 column (4.6 mm×250 mm
5 μm). The mobile phase was consisted of methanol and water (55 ∶45). Detection wavelength was at 280 nm. The flow rate was set at 1.0 mL ·min-1. Result: A small amount of arctiin and no arctigenin was found in the root; while stem and leaf nearly did not contain. Leaves and inflorescence did not contain arctiin and a few arctigenin. But both ingredients were higher in the fruit than in the leaves and inflorescence. Conclusion: The method was simple and accurate
and it could be used for simultaneous determination of arctiin and arctigenin from Radix Arctium
to provide the basis for a better development of A. tomentosum Mill.