ZHOU Nong, ZOU Liang, WANG Guang-zhi, et al. Primary Explore to Relation of Arbuscular Mycorrhizae and its Secondary Metabolite Steroidal Saponin in [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 85-88.
ZHOU Nong, ZOU Liang, WANG Guang-zhi, et al. Primary Explore to Relation of Arbuscular Mycorrhizae and its Secondary Metabolite Steroidal Saponin in [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 85-88.DOI:
Objective: To investigate the correlation between the content of its secondary metabolites
steroidal saporins and the infection ratio and spore density of arbuscular mycorrhizal fungi of P. polyphylla. Method: By Phillips and Hayman staining
the arbuscular mycorrhizal of P. polyphylla were selected
and by Gendemanns Wet-screening method
the AMF spores were obtained and counted.Meanwhile
content of four steroidal saporins (paris saporinⅠ
Ⅱ
Ⅵ
Ⅶ) were determined by HPLC. Result: Through mathematic model analysis
the infection ratio showed conspicuous positive correlation with the spore density of mycorrhizal structures of P. polyphylla
while the infection ratio and spore density showed some positive correlation with the total steroidal saporin content in the rhizome. Conclusion: Arbuscular mycorrhizal fungi may be an important factor which affect the content of active moiety in P. polyphylla.