Objective: To study the extraction and assaying of Safflower polysaccharide (SPS)

and to provide suitable experimental drug specimen for exploreing the anti-carcinoma effect of SPS. Method: Water extracting and ethanol precipitating methods were applied to extract polysaccharide from Safflower. By Sevage method

protein can be removed

and by oxidation method to decolor the polysaccharide. After phenol-sulphuric acid coloring

the polysaccharide content was determined by the method of absorption spectrometry. Result: The standard curve was liner in the concentration range of 5~50 μg·mL-1 with correlation coefficient of r=0.9994

the linear regression equation was Y=0.01749X-0.03165

the contents of polysaccharide from Safflower was 96.048%. Conclusion: This methods have advantages of simple operation

good stability

high accuracy and good reproducibility.This extraction technology could be used to extract Safflower polysaccharide for studies on its anti-carcinoma effect.