Objective: To establish the quality standard of Huanglianjiedu Hydrogel. Method: The high performance liquid chromatography was developed for the determination the contention of jasminoidin

berberine and baicalin;Luna C18 column(4.6 mm×250 mm

5 μm) was used in the determination and the mobile phase of jasminoidin

berberine and baicalin were a mixture of acetonitrile-water(13 ∶87)

acetonitrile-0.1 mol·L-1 K2HPO4(30 ∶70)

and methanol-water-phosphoric acid (47 ∶53 ∶0.2) respectively. Result: The average content of jasminoidin was no less than 0.4%

and the average recovery and RSD were 97.21% and 1.5% respectively; The average content of berberine was no less than 1.2%

and average recovery and RSD were 99.11% and 2.45% respectively; The average content of jasminoidin was no less than 1.4%

and the average recovery and RSD were 98.74% and 1.6% respectively; Conclusion: This method can be adopted as a standard for the quality control of Huanglianjiedu Hydrogel.