Objective: The aim of this study was to isolate the endophyte of Ficus carica and screen of its antibiotic activities. Method: The endophytic fungi were indentified by morphology

Staphylococcus aureus

Escherichia coli

Alternaria brassicae

Valsa mali

Fusarium graminearum

F. oxysporumf. sp. fragariae

Penicillium notatum were selected as the indicator to test the inhibitory activity of the isolated strains. Result: Sixty-one strains of endophytic fungi were isolated from the healthy root

stem and leaf of F. carice

which belonged to twenty genera

twelve families

seven orders and five classes. Of them forty strains were antimicrobial. The percentage of antimicrobial strains to tested strains is 65.57%. Conclusion: It indicated that the endophyte of F. carica were diversity and rich and has the best research and application value.