Objective: To determine the content of aloe-emodin

rhein

emodin

chrysophanol and physcion in Yankening Tablets by HPLC. Method: Using Kromasil C18 column (4.6 mm×250 mm

5 μm)

the methanol(A)-0.1%phosphoric acid (B)was used as the mobile phase. Using gradient elution

0~10 min

75%~85% A

10~20 min

85%~90% A. The flow rate was 1.0 mL/min. The column temperature was 25 ℃ and the detection wavelength was 254 nm. Result: The linearity of this method were for 0.016-0.0960 mg

0.120-0.072 0 μg for rhein

0.016 0-0.096 0 μg for emodin

0.036 4-0.218 4 μg for chrysophanol

0.015 6-0.093 6 μg for physcion. There coveries of aloe-emodin

rhein

emodin

chrysophanol and physcion were 96.6%

97.2%

96.9%

98.1% and 96.2%. RSD were 0.76% (n=5)

1.35% (n=5)

1.57% (n=5)

1.25% (n=5) and 0.62% (n=5)

respectively. Conclusion: This method was rapid

accurate and can be used as one kind of Yankening Tablets’ quality control method.