MA Qun1, LU Jian-qiu1, DU Jing2, et al. HPLC Determination of sophoricoside in Diyuhuaijiao Pills[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(8): 12-13.
MA Qun1, LU Jian-qiu1, DU Jing2, et al. HPLC Determination of sophoricoside in Diyuhuaijiao Pills[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(8): 12-13.DOI:
Objective:To establish an HPLC method for determiantion of sophoricoside in Diyuhuaijiao pills.Method:Zorbax C18(4.6×150 mm
5 μm) column was adopted with methanol-acetonitrile-0.1% phosphoric acid(1∶10∶79) as the mobile phase at 0.8 mL·min-1.The detection wave length was 260 nm and column temperature was 40 ℃.Results:The average recovery was 99.21% and the relative standard deviation(RSD)was 0.62%.Conclusion:The method is simple
fast
sensitive and can be used for the quality control of Diyuhuaijiao pills.