Objective:To determine the content of Emodin

Chrysophanol in Yankening tablets by high performance liquid chromatography.Methods:The determination was carried out on a Shim-pack-C18 column(5 μm

150 mm×4.6 mm)eluted with the mobile phase of methanol water containing 0.1% phosphoric acid(85∶15).The detection wavelength was 254 nm.Results:A good linear relationship between the peak areas and the injection amounts was found for emodin within(0.016 128～0.080 64)μg

with a correlation coefficient of 0.999 7

for chrysophanol within(0.032 48～0.162 4)μg

with a correlation coefficient of(0.999 8).The average recovery of Emodin was 97.88%(RSD 1.64%)

and of Chrysophanol was 97.93%(RSD 0.71%).Conclusion: The method is simple

accurate

sensitive and can be applied to the quality control of the preparation.