HPLC法同时测定新疆一枝蒿中木犀草素和一枝蒿酮酸的含量

张洁1; 顾政一2; 何承辉2

您当前的位置：

首页 >

文章列表页 >

HPLC法同时测定新疆一枝蒿中木犀草素和一枝蒿酮酸的含量

更新时间：2024-01-04

- HPLC法同时测定新疆一枝蒿中木犀草素和一枝蒿酮酸的含量
- Determination of Luteolin and Rupestonic Acid in by High Performance Liquid Chromatography
- 中国实验方剂学杂志 2009年15卷第4期页码：1-3
- 作者机构：
- 作者简介：
- 基金信息：
  
  新疆维吾尔自治区高技术研究发展计划项目(200711105)
- DOI：
  中图分类号：
- 纸质出版日期：2009
- 稿件说明：
移动端阅览
张洁1, 顾政一2, 何承辉2. HPLC法同时测定新疆一枝蒿中木犀草素和一枝蒿酮酸的含量[J]. 中国实验方剂学杂志, 2009,15(4):1-3.

ZHANG Jie1, GU Zheng-yi2, HE Cheng-hui2. Determination of Luteolin and Rupestonic Acid in by High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(4): 1-3.
张洁1, 顾政一2, 何承辉2. HPLC法同时测定新疆一枝蒿中木犀草素和一枝蒿酮酸的含量[J]. 中国实验方剂学杂志, 2009,15(4):1-3. DOI：

ZHANG Jie1, GU Zheng-yi2, HE Cheng-hui2. Determination of Luteolin and Rupestonic Acid in by High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(4): 1-3. DOI：

摘要

目的:建立新疆一枝蒿中的主要有效成分木犀草素与一枝蒿酮酸的高效液相含量测定方法。方法:采用高效液相色谱法测定

Shim packODSC18(4.6mm×250mm

5μm);甲醇-0.4%磷酸(45∶55)

检测波长350nm

该条件下测定木犀草素;检测波长241nm

该条件下测定一枝蒿酮酸。结果:木犀草素在12.80～51.20μg·mL-1范围内

峰面积与进样浓度具有良好线性关系

r=0.9998

平均回收率为99.92%(n=9);一枝蒿酮酸在9.72～77.76μg·mL-1范围内线形关系也良好

r=0.9993

平均回收率为99.82%(n=9)。结论:本法专属性强

操作简便

结果准确。适用于检测新疆一枝蒿中木犀草素和一枝蒿酮酸的含量。

Abstract

Objective:To develop an HPLC method for detecting luteolin and rupestonic acid

two key active constituents of Artemisia rupestis L.Method:A Shimpack ODSC18 column(4.6 mm×250 mm

5 μm) was used.The mobile phase was methanol:0.4% phosphoric acid(45∶55).The detection wavelengths were at 350 nm for luteolin

and 241 nm for rupestonic acid

respectively.Result: For luteolin

the linearity was obtained over the range of 12.80～51.20 μg·mL-1 with r=(0.999 8).The average recovery was 99.92%(n=9);for rupestonic acid

the linearity was obtained over the range of 9.72～77.76 μg·mL-1 with r=(0.999 3).The average recovery was 99.82%(n=9).Conclusion: The method was proved to be simple

rapid

and reliable to determine the contents of luteolin and rupestonic acid in Artemisa rupestis.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

HPLC同时测定裸花紫珠药材中毛蕊花糖苷和木犀草素

HPLC测定黑草中木犀草素和芹菜素的含量

冻干与生晒西洋参饮片差异皂苷含量及促斑马鱼血管生成活性比较

经典名方二冬汤基准样品的指标成分含量测定及量值传递规律探索

马鞭草中6个肝毒性吡咯里西啶生物碱的含量测定及风险评估